The method utilizes an immunized germfree animal.

该方法使用一种免疫的无菌动物。

Left sciatic nerves were cut down on germfree conditions and given primary suture of epineurium.

无菌条件下切断左侧坐骨神经，一期神经外膜缝合。

Stressing the training of the germfree operation and raising the operation skill are important to lower the pollution rate.

注重无菌操作的训练，提高操作水平是降低污染率的重要手段。

Method: We adopted germfree caesarean section and artificial suckling to make CV New Zealand Laboratorial rabbit biology-cleansing and cultivate SPF New Zealand Laboratorial rabbit.

方法：采用无菌剖腹产与人工哺乳技术，对CV级新西兰实验兔进行了微生物和寄生虫净化，培育出SPF级新西兰实验兔；