Interestingly, both mutant alleles had a single-base deletion, which was confirmed by PCR-RFLP.

有趣的是两个突变体中都发生了单个碱基的缺失，PCR-RFLP证实了该结果。

Currently, T_RFLP rarely applied in China, and it has no applications on microbial community analysis of nitrifying bacteria.

但作为一种研究微生物群落结构特征的理想方法，将会得到广泛地应用。

Plasmon PCR-RFLP technology can therefore be used to detect both interspecific and intraspecific genetic variations in Kengyilia.

因此，利用细胞质基因组PCR-RFLP标记不仅可检测到仲彬草属种间多态性，也可检测到种内多态性。

Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.

方法：采用PCR技术和限制性内切酶片段长度多态性（RFLP）的方法，检测中国北方汉族168个核心家系的基因型。

Experimental results based on backcross population indicated that the sensitivity and specificity of PCR marker were almost as same as that of RFLP marker.

回交后代的鉴定结果表明，该PCR标记的灵敏度和专化性与RFLP标记基本相同。

RFLP shown that, in the 33 clones of patient D , there were 32 clones were YMDD of pre-therapy and 29 clones were YIDD of post-therapy sample, except land 4 synonymous mutations, respectively.

限制性片段长度多态性分析结果表明病人D治疗前33个克隆中32个为YMDD，治疗后33个中有29个克隆是YIDD，余下的为其它同义突变。