过度搅拌可能会变性蛋白质。

Overmixing can denature the proteins.

加热会变性蛋白质。

Heating will denature the protein.

蛋白折叠液相色谱法（PFLC）用于变性蛋白质复性并同时纯化时对流动相组成及其洗脱条件的要求远较通常的液相色谱法高。

Optimization of mobile phase composition in PFLC for protein renaturation with simultaneous purification is thus much more important than that in usual liquid chro.