A CIK cell in-vitro culture method is disclosed.

本发明公开了一种CIK细胞体外培养方法。

Objective:To research PHA-CIK cells' phenotype and cytotoxicity .

目的：探讨PHA-CIK细胞的免疫表型和细胞毒活性。

Objective: to investigate the level of cytokine excreted by CIK cells in vitro.

目的：探讨CIK细胞在培养诱导过程中分泌的细胞因子水平。

Objective To discuss the variety developments of the immune phenotype of CIK in vitro.

目的探讨体外培养过程中CIK细胞的细胞免疫表型的动态变化。

This paper is our attempt to summarize the available published literature related to CIK cells.

这份报告的目的意在探讨关于CIK细胞的有效的公开性文献。

CIK cell is a kind of anti-vims cell and is an important immunocyte to kill the tumor cell directly.

淋巴细胞为主，CIK细胞是抗病毒、直接杀伤肿瘤细胞的主要免疫细胞。

Aim: to study the effect of phytohemagglutinin (PHA) on cytokine induced killers (CIK) morphology and immunophenotype.

目的：探讨植物血凝素(PHA)对细胞因子诱导的杀伤细胞(CIK)形态和免疫表型的影响。

Objective to investigate the cytotoxicity and mechanism of killing tumor of cytokine-induced killer (CIK) cells in vitro.

目的探讨细胞因子诱导的杀伤细胞(CIK)体外杀伤活性及杀瘤机制。

Objective: to study the effect of phytohemagglutinin (PHA) on cytokine induced killers' (CIK) proliferation and cytotoxicity.

目的：探讨植物血凝素(PHA)对细胞因子诱导的杀伤细胞(CIK)增殖和细胞毒作用的影响。

Our laboratory has got the abundant experience to CIK research, and has cured more than 400 tumor patients successfully with CIK So far.

本实验室对CIK细胞的研究有着丰富的经验，到目前为止已成功地用CIK细胞治疗了400多例的肿瘤患者，并有深入研究。

Objective to observe the therapeutic effect of autologous cytokine-induced killer cells (CIK) on HBV DNA positive patients with liver cirrhosis.

目的观察自体细胞因子诱导杀伤细胞(CIK细胞)治疗HBV DNA阳性肝硬化患者的近期疗效。

Conclusion:The combined treatment of DC and CIK is effective in curing Leukemia patients as a new immunology treatment of acute Leukemia and has potential clinical prospect.

结论：DC与CIK联合治疗急性白血病安全有效，为急性白血病提供了一种新的有效的免疫治疗手段，具有潜在的临床应用前景。

The cell used in adoptive immunotherapy must have strong cytotoxic and proliferative ability, so CIK cell is thought of the new generation anti-tumor adoptive immunity treatment.

用于过继免疫治疗的活性细胞必须具有较强的细胞活性和增殖力，CIK细胞被认为是新一代抗肿瘤过继细胞免疫治疗的首选方案。

CONCLUSION: CIK cells have strong proliferative ability and higher cytotoxicity to tumor cells in vitro, which could be used as a potential anti-tumor adoptive immunotherapy in clinic.

结论：CIK细胞在体外扩增能力强，对肿瘤细胞的杀伤活性高，有望成为新一代抗肿瘤过继免疫细胞制剂而应用于临床。

CONCLUSION: CIK cell transfusion after micro-invasive treatments may improve the immunologic function in HCC patients, and play an important role in reducing the recurrence rate of HCC.

结论：微创治疗后联合cik细胞免疫治疗可提高肝癌患者的免疫功能，对降低肝癌的复发率具有重要作用。

Hence, dendritic cells (DC) were cultured and engineered to present tumor antigens to CIK cells hoping that this might enhance the recognition of tumor cells and its subsequent killing.

因此，树突状细胞(DC细胞)递呈肿瘤抗原给共培养的CIK细胞，希望可以增强CIK细胞识别肿瘤的能力以及提高其肿瘤杀伤活性。

Methods Co-culture tumor antigen-sensitized dendritic cells (DCs) with cytokine induced killer (CIK) cells, analyze the immune epitope and determine the killing effect on tumor cells by MTT method.

方法采用肿瘤抗原致敏的DC与CIK细胞共培养，分析其免疫表型，并用mtt染色法检测其对肿瘤细胞的杀伤力。