RESULTS: Antisense VEGF gene eukaryotic expression vector was successfully constructed.

结果： 成功构建反义VEGF基因真核表达载体；

Conclusion: pED GCSF is a high eukaryotic expression plasmid in mammalian cell.

结论: pED-GCSF是一个能在哺乳动物细胞中高效表达rhG-CSF的真核表达质粒.

CB could be expressed in high yield by vaccinia virus eukaryotic expression system.

痘苗病毒真核表达系统可以高效表达目的蛋白.

To construct the eukaryotic expression vector of GGT1 and detect its localization in COS7 cells.

构建GGT1重组真核表达载体，观察GGT1在COS7细胞中的定位。

Methods An eukaryotic expression vector encoding Hantaan virus S gene coding region was constructed.

方法利用基因重组技术,构建含汉滩病毒S片段编码区基因的真核表达质粒.

Objective To construct eukaryotic expression vector of human pancreatic duodenal homeobox 1(PDX-1) gene, and to detect its expression in NIH3T3 cell lines.

目的构建人胰十二指肠同源盒（PDX-1）基因的真核表达载体，观察其在NIH3T3细胞中的表达。

Objective:To construct an eukaryotic expression vector containing the open reading frame (ORF) of long type of LRP16 gene and to detect its expression in HL60 cells.

目的：构建长型LRP16基因开放阅读框架序列的真核表达载体，并观察其在髓系白血病细胞系HL6 0细胞中的表达情况。

In view of Pichia Pastoris expression system is eukaryotic expression system which has modification process such as posttranslational processing, we prefer to express gene in it.

考虑到毕赤酵母表达系统属于真核表达系统，具备翻译后加工等一系列修饰过程，首选其进行表达。

The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.

实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。

Objective To construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.

目的建立丙型肝炎病毒（HCV）复合多表位基因的真核表达载体，并在COS7细胞中瞬时表达。