Methods Peripheral blood mononuclear cells(PBMC) were collected by using lymphocyte Ficoll and the low-osmotic hemolysis method.

方法用淋巴细胞分层法和低渗溶血法提取PBMC。

This paper reports the successful isolation of islets of Langerhans from the rat pancreas using a modified collagenase digestion, Ficoll density gradient separation and hand-picking technique.

用改良胶原酶消化技术，聚蔗糖密度梯度离心及手法挑拾方法从大鼠胰腺成功地分离出完整的胰岛。