Conclusion: ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.

结论：在复苏后早期阶段ES细胞培养中，不宜单独应用BRL条件培养基，须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层。

Objective: In order to offer evidence for the clinical application of HM(harmine), toxicity of HM and HCPT(hydroxycamptothecin) against the rat normal liver cell (BRL) was compared.

目的：通过去氢骆驼蓬碱 （HM）微球与羟基喜树碱 （HCPT）注射液对大鼠正常肝细胞 （BRL ）的毒性作用的比较研究 ，为 HM的临床应用提供佐证。