Serum-free DMEM medium with high glucose works for us.
我们使用的是不含血清的高糖DMEM培养基,效果很好。
Objective To compare the culture effects of CHO-C28 cells by domestic and imported DMEM.
目的比较国产与进口DMEM培养基培养cho - C28细胞的效果。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
在D MEM培养基中进行了原代及传代培养,对其中的成纤维细胞进行分离纯化。
The cells were planked with lower cell density, and further screened in DMEM medium containing G418 MFD;
采用较低的细胞密度铺板,加入含最小致死量G418的DMEM培养基进行再次筛选;
The friction force of PGA porous scaffolds between DMEM is increases with the increase of the flow velocity.
聚羟基乙酸PGA管状支架的摩擦力随流速的增加而增加。
The amounts of living cells and melanin of mouse melanoma B16 cultured in DMEM and RPMI1640 media are tested.
对小鼠黑色素瘤B1 6细胞在DMEM和RPMI1 640两种培养基中培养产生的活细胞数和黑色素量进行了检测。
The cultured HUVECs were divided into 6 groups according to different experimental agents: control ( serum - free DMEM ) ;
施加实验因素,分成6组:正常对照组(无血清DMEM);
Results: (1) Sub-confluent cells having starved in DMEM containing 0.4% FBS for 48h, 88.5% of the cells were at G0/G1 phase of the cell cycle.
研究结果表明:(1)培养至次融汇状态的乳鼠心脏成纤维细胞经低血清(0.4%FBS) 饥饿培养48小时,G0/G1期细胞占88.5%;
Conclusion Domestic DMEM was safe and effective for cell culture and reached the level of imported DMEM, thus was suitable for large-scale production of HB vaccine.
结论国产培养基在细胞培养中安全、有效,达到进口培养基水平,可用于规模化生产。
After 3 passages were obtained, and then digested by DMEM medium containing fetal calf serum, cell amount was counted and the proliferation curve of MSCs was depicted.
取第3代骨髓基质细胞,以含胎牛血清的DMEM培养液培养消化后,计数细胞量,描绘细胞增殖曲线。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养,观察细胞增殖及传代效果,确定无血清液体培养基的营养功能。
After the human umbilical mesenchymal stem cells were treated with neuronal conditioned medium (NCM) for 9 days, the gene expression groups are compared to those only treated with DMEM.
在人脐间质干细胞治疗神经细胞条件培养基(NCM)为9天,该基因表达组相比,仅治疗液。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法:采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞,并用含10%优等胎牛血清的H - DMEM培养液培养。
Meanwhile, normal NIH3T3 cells were taken as controls, and cultured by G418 contain MFD and by DMEM medium without G418 respectively. Light microscope was used to observe G418R NIH3T3 cells.
同时选用正常的NIH3T3细胞为对照,使用含有最小致死量g418及未加g 418的DMEM培养基进行培养,采用光学显微镜对G418抗性NIH3T3细胞进行观察。
A low calcium medium developed for epidermal keratinocytes were prepared according to the MCDB 153 modified formula and used in human epidermal keratinocyte culture compared with DMEM culture system.
作者参考MCDB 153培养基配方,以DMEM培养系统作为对照,开展了人表皮细胞低钙培养基的配制和对人表皮细胞培养的研究。